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sec-deficient strains  (Seca)

 
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    Structured Review

    Seca sec-deficient strains
    <t>TssL</t> insertion is dependent upon YidC and DnaK. Soluble proteins (soluble and peripherally associated membrane proteins; S) and integral inner membrane proteins (IM) were collected from various genetic backgrounds (WT-1, MC4100; secA , MM52 grown in restrictive conditions; <t>secB</t> , A443; tat , DADE; ftsY , IY28 grown in restrictive conditions; yidC , FTL10 grown in restrictive conditions; yidC + , FTL10 grown in permissive conditions; WT-2, BW25113; dnaKJ , PK101; dnaJ , GP108; dnaJ djlA , GP110; tig , A1091) and subjected to 12.5% acrylamide SDS-PAGE. The localization of the TolR and Flag TssL proteins was assessed by immunodetection using anti-TolR and anti-FLAG antibodies, respectively. Arrows indicate soluble fractions of strains in which the TssL protein is not properly inserted in the inner membrane. Molecular weight markers are indicated on the left.
    Sec Deficient Strains, supplied by Seca, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sec-deficient strains/product/Seca
    Average 90 stars, based on 1 article reviews
    sec-deficient strains - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC"

    Article Title: The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC

    Journal: MicrobiologyOpen

    doi: 10.1002/mbo3.9

    TssL insertion is dependent upon YidC and DnaK. Soluble proteins (soluble and peripherally associated membrane proteins; S) and integral inner membrane proteins (IM) were collected from various genetic backgrounds (WT-1, MC4100; secA , MM52 grown in restrictive conditions; secB , A443; tat , DADE; ftsY , IY28 grown in restrictive conditions; yidC , FTL10 grown in restrictive conditions; yidC + , FTL10 grown in permissive conditions; WT-2, BW25113; dnaKJ , PK101; dnaJ , GP108; dnaJ djlA , GP110; tig , A1091) and subjected to 12.5% acrylamide SDS-PAGE. The localization of the TolR and Flag TssL proteins was assessed by immunodetection using anti-TolR and anti-FLAG antibodies, respectively. Arrows indicate soluble fractions of strains in which the TssL protein is not properly inserted in the inner membrane. Molecular weight markers are indicated on the left.
    Figure Legend Snippet: TssL insertion is dependent upon YidC and DnaK. Soluble proteins (soluble and peripherally associated membrane proteins; S) and integral inner membrane proteins (IM) were collected from various genetic backgrounds (WT-1, MC4100; secA , MM52 grown in restrictive conditions; secB , A443; tat , DADE; ftsY , IY28 grown in restrictive conditions; yidC , FTL10 grown in restrictive conditions; yidC + , FTL10 grown in permissive conditions; WT-2, BW25113; dnaKJ , PK101; dnaJ , GP108; dnaJ djlA , GP110; tig , A1091) and subjected to 12.5% acrylamide SDS-PAGE. The localization of the TolR and Flag TssL proteins was assessed by immunodetection using anti-TolR and anti-FLAG antibodies, respectively. Arrows indicate soluble fractions of strains in which the TssL protein is not properly inserted in the inner membrane. Molecular weight markers are indicated on the left.

    Techniques Used: Membrane, SDS Page, Immunodetection, Molecular Weight



    Similar Products

    sec-deficient strains  (Seca)
    90
    Seca sec-deficient strains
    <t>TssL</t> insertion is dependent upon YidC and DnaK. Soluble proteins (soluble and peripherally associated membrane proteins; S) and integral inner membrane proteins (IM) were collected from various genetic backgrounds (WT-1, MC4100; secA , MM52 grown in restrictive conditions; <t>secB</t> , A443; tat , DADE; ftsY , IY28 grown in restrictive conditions; yidC , FTL10 grown in restrictive conditions; yidC + , FTL10 grown in permissive conditions; WT-2, BW25113; dnaKJ , PK101; dnaJ , GP108; dnaJ djlA , GP110; tig , A1091) and subjected to 12.5% acrylamide SDS-PAGE. The localization of the TolR and Flag TssL proteins was assessed by immunodetection using anti-TolR and anti-FLAG antibodies, respectively. Arrows indicate soluble fractions of strains in which the TssL protein is not properly inserted in the inner membrane. Molecular weight markers are indicated on the left.
    Sec Deficient Strains, supplied by Seca, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sec-deficient strains/product/Seca
    Average 90 stars, based on 1 article reviews
    sec-deficient strains - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    TssL insertion is dependent upon YidC and DnaK. Soluble proteins (soluble and peripherally associated membrane proteins; S) and integral inner membrane proteins (IM) were collected from various genetic backgrounds (WT-1, MC4100; secA , MM52 grown in restrictive conditions; secB , A443; tat , DADE; ftsY , IY28 grown in restrictive conditions; yidC , FTL10 grown in restrictive conditions; yidC + , FTL10 grown in permissive conditions; WT-2, BW25113; dnaKJ , PK101; dnaJ , GP108; dnaJ djlA , GP110; tig , A1091) and subjected to 12.5% acrylamide SDS-PAGE. The localization of the TolR and Flag TssL proteins was assessed by immunodetection using anti-TolR and anti-FLAG antibodies, respectively. Arrows indicate soluble fractions of strains in which the TssL protein is not properly inserted in the inner membrane. Molecular weight markers are indicated on the left.

    Journal: MicrobiologyOpen

    Article Title: The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC

    doi: 10.1002/mbo3.9

    Figure Lengend Snippet: TssL insertion is dependent upon YidC and DnaK. Soluble proteins (soluble and peripherally associated membrane proteins; S) and integral inner membrane proteins (IM) were collected from various genetic backgrounds (WT-1, MC4100; secA , MM52 grown in restrictive conditions; secB , A443; tat , DADE; ftsY , IY28 grown in restrictive conditions; yidC , FTL10 grown in restrictive conditions; yidC + , FTL10 grown in permissive conditions; WT-2, BW25113; dnaKJ , PK101; dnaJ , GP108; dnaJ djlA , GP110; tig , A1091) and subjected to 12.5% acrylamide SDS-PAGE. The localization of the TolR and Flag TssL proteins was assessed by immunodetection using anti-TolR and anti-FLAG antibodies, respectively. Arrows indicate soluble fractions of strains in which the TssL protein is not properly inserted in the inner membrane. Molecular weight markers are indicated on the left.

    Article Snippet: We did not observe TssL mis-localization in Sec-deficient ( secA , secB ) or SRP-deficient ( ftsY ) strains.

    Techniques: Membrane, SDS Page, Immunodetection, Molecular Weight